Candelario, Kate M.;Shuttleworth, C. William;Cunningham, Lee Anna published 《Neural stem/progenitor cells display a low requirement for oxidative metabolism independent of hypoxia inducible factor-1alpha expression》 in 2013. The article was appeared in 《Journal of Neurochemistry》. They have made some progress in their research.Product Details of 329-89-5 The article mentions the following:
Neural stem/progenitor cells (NSPCs) are multipotent cells within the embryonic and adult brain that give rise to both neuronal and glial cell lineages. The maintenance of NSPC multipotency is promoted by low O2 tension, although the metabolic underpinnings of this trait have not been described. Here, the authors investigated the metabolic state of undifferentiated NSPCs in culture, and tested their relative reliance on oxidative vs. glycolytic metabolism for survival, as well as their dependence on hypoxia inducible factor-1α (HIF-1α) expression for maintenance of metabolic phenotype. Unlike primary neurons, NSPCs from embryonic and adult mice survived prolonged hypoxia in culture. In addition, NSPCs displayed greater susceptibility to glycolytic inhibition compared with primary neurons, even in the presence of alternative mitochondrial TCA substrates. NSPCs were also more resistant than neurons to mitochondrial CN– toxicity, less capable of utilizing galactose as an alternative substrate to glucose, and more susceptible to pharmacol. inhibition of the pentose phosphate pathway by 6-aminonicotinamide. Inducible deletion of exon 1 of the Hif1a gene improved the ability of NSPCs to utilize pyruvate during glycolytic inhibition, but did not alter other parameters of metabolism, including their ability to withstand prolonged hypoxia. Taken together, these data indicate that NSPCs have a relatively low requirement for oxidative metabolism for their survival and that hypoxic resistance is not dependent upon HIF-1α signaling.6-Aminonicotinamide (cas: 329-89-5) were involved in the experimental procedure.
6-Aminonicotinamide (cas:329-89-5)Product Details of 329-89-5 is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.
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