Category: amides-buliding-blocksIn 2021, Hasan, Mahmudul Md.;Tasmin, Most. Sayla;El-Shehawi, Ahmed M.;Elseehy, Mona M.;Reza, Abu Md.;Haque, Ariful published 《R. vesicarius L. exerts nephroprotective effect against cisplatin-induced oxidative stress》. 《BMC Complementary Medicine and Therapies》published the findings. The article contains the following contents:
Cisplatin is an outstanding anticancer drug, but its use has been decreased remarkably due to sever nephrotoxicity. R. vesicarius L. is a leafy vegetable that is evident with anti-angeogenic, anti-inflammatory, anti-proliferative, hepatoprotective, and nephroprotective potential. Therefore, this study was designed to inspect its methanol extract (RVE) for possible nephroprotective effect. Primarily, in vitro antioxidant activity of RVE was confirmed based on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging aptitude. Thereafter, Swiss Albino male mice were treated with cisplatin (2.5 mg/kg) for 5 successive days to induce nephrotoxicity. Recovery from nephrotoxicity was scrutinized by treating the animals with RVE (25, 50, and 100 mg/kg) i.p. (i.p.) for the next 5 consecutive days. After completion of treatment, mice were sacrificed and kidneys were collected. Part of it was homogenized in sodium phosphate buffer for evaluating malondialdehyde (MDA) level, another part was used to evaluate gene (NQO1, p53, and Bcl-2) expression. Moreover, the hydrogen peroxide (H2O2) neutralizing capacity of RVE was evaluated in HK-2 cells in vitro. Finally, bioactive phytochems. in RVE were determined using gas chromatog.-mass spectrometry (GC-MS). RVE showed in vitro antioxidant activity in a dose-dependent fashion with 37.39 ± 1.89 μg/mL IC50 value. Treatment with RVE remarkably (p < 0.05) decreased MDA content in kidney tissue. Besides, the expression of NQO, p53, and Bcl-2 genes was significantly (p < 0.05) mitigated in a dose-dependent manner due to the administration of RVE. RVE significantly (p < 0.05) reversed the H2O2 level in HK-2 cells to almost normal. From GC-MS, ten compounds including three known antioxidants “4H-Pyran-4-one, 2, 3-dihydro-3,5-dihydroxy-6-methyl-“, “Hexadecanoic acid”, and “Squalene” were detected. The extract was rich with an alkaloid “13-Docosenamide”. Overall, RVE possesses a protective effect against cisplatin-induced kidney damage. And cis-13-Docosenamide (cas: 112-84-5) was used in the research process.
cis-13-Docosenoamide(cas: 112-84-5) is a primary fatty amide resulting from the formal condensation of the carboxy group of erucic acid with ammonia.Category: amides-buliding-blocks It is commonly used as a slip additive in the plastic manufacturing industry.
Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics