Duan, Y.;Chen, J.;Pang, Z.;Ye, X.;Zhang, C.;Hu, H.;Xie, J. published 《Antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana》 in 2021. The article was appeared in 《Journal of Applied Microbiology》. They have made some progress in their research.Recommanded Product: cis-13-Docosenamide The article mentions the following:
In this study, antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana was investigated. Methods and Results : The physiol. strain of banana fusarium pathogen Fusarium oxysporum f. sp. cubense Race 4 (FOC.4) was used as the target fungus, and the antifungal mechanism of the crude extract of Streptomyces ma. FS-4 was investigated. Eighteen different compounds identified by gas chromatog.-mass spectrometry were composed of aldehydes, Me, hydrocarbons, amides, esters and acids. FS-4 significantly inhibited the spore germination of the target fungi, with an EC50 of 22·78 μg ml-1. After treatment with 100 μg ml-1 FS-4 crude extract, the N-acetylglucosamine content in the mycelium increased 1·95-fold. However, the extract had no significant effect on β-1,3-glucanase. At the FS-4 crude extract dose of 100 μg ml-1, the total sugar and protein contents decreased by 28·6 and 29·1% resp., and the fat content was 41·3%. FS-4 significantly inhibited the activity of the mitochondrial complex III of Foc4, which was reduced by 52·45%. Moreover FS-4 reduced the activity of succinate dehydrogenase, a key enzyme in the Krebs cycle, by 60·2%. However, FS-4 had no significant effect on malate dehydrogenase. The membrane potential on the mitochondrial inner membrane was significantly reduced at the test concentration of 100 μg ml-1. ROS gradually accumulated in the Foc4 hypha, and the burst was 3·97 times higher than the control. To complete the study, the researchers used cis-13-Docosenamide (cas: 112-84-5) .
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Reference:
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