Zhou, Chuan;Ma, Dianping;Cao, Wenming;Shi, Haiming;Jiang, Yuanrong published 《Fast simultaneous determination of capsaicin, dihydrocapsaicin and nonivamide for detecting adulteration in edible and crude vegetable oils by UPLC-MS/MS》 in 2018. The article was appeared in 《Food Additives & Contaminants, Part A》. They have made some progress in their research.Safety of N-Vanillylnonanamide The article mentions the following:
Capsaicinoids are pungent components in hot peppers, which have been detected in waste cooking oil. However, trace anal. of capsaicinoids in edible and crude vegetable oils is a challenging task due to the complex matrix. In this study, a simple liquid-liquid extraction and solid phase extraction (SPE) coupled with RP-UPLC-ESI-MS/MS method was developed for the quantification of capsaicinoids in edible and crude vegetable oils to screen the adulteration with waste cooking oil. This method was used to simultaneously determine 3 capsaicinoids (capsaicin, dihydrocapsaicin, and nordihydrocapsaicin) with capsaicin-d3, and dihydrocapsaicin-d3 as internal standards This method allows the complete anal. of a sample in only an hour, even including sample preparation and chromatog. separation The linear range of 3 capsaicinoids ranged between 0.5 and 40 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) for capsaicinoids were calculated as 0.15 and 0.5 μg/kg, resp. Quant. recoveries ranging from 92.9% to 105% were obtained by the anal. of spiked oil. The relative standard deviations were less than 5% (n = 6). The established method can potentially overcome the interference of triacylglycerols and fatty acids in edible and crude vegetable oils, and have been successfully applied to analyze real oil samples. This method provided a rapid and reliable method for the detection of adulteration of vegetable oils with waste cooking oils.N-Vanillylnonanamide (cas: 2444-46-4) were involved in the experimental procedure.
N-Vanillylnonanamide(cas:2444-46-4) is a capsaicin analog that has been used to study the effects of capsaicin on energy metabolism and bowel disease.Safety of N-Vanillylnonanamide It has been shown to be effective in the treatment of bowel disease, by inhibiting the production of proinflammatory cytokines and prostaglandins.
Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics