Name: N,2-DihydroxybenzamideIn 2021, Dey, Tanmay;Das, Satyajit;Majumdar, Arkajo;Kar, Rup Kumar published 《Apoplastic reactive oxygen species mediated escape growth of root during illumination in Vigna radiata (L.) Wilczek seedlings》. 《Acta Physiologiae Plantarum》published the findings. The article contains the following contents:
Besides gravity, roots are also guided by light to grow deep into the soil and sensitivity of roots to light is evidently due to presence of photoreceptors like phototropins. Such light-induced root growth (light-escape growth) presumably involves reactive oxygen species (ROS). Present study explores the possibility of ROS action in this event during early seedling growth of Vigna radiata based on pharmacol. evidences. Germinated (20 h) seeds were incubated in dark or light in presence of general ROS scavenger (Pr gallate), specific scavengers of O·2– (copper chloride; CuCl2), H2O2 [dimethylthiourea (DMTU) and potassium iodide (KI)] and ·OH (sodium benzoate) and ROS-producing enzyme inhibitors [zinc chloride (ZnCl2), inhibitor of NADPH oxidase (NOX); diethyldithiocarbamate (DEDTC), inhibitor of superoxide dismutase (SOD) and salicylhydroxamic acid (SHAM), inhibitor of peroxidase]. Light-induced root growth of 3-day seedlings diminished signifcantly in case of all the treatments suggesting for a pos. role of ROS in light-escape growth. This is supported by elevated level of apoplastic ROS in light grown roots as evident from ROS-specific staining [nitroblue tetrazolium chloride (NBT) for O·2– and 3,3,5,5-tetramethylbenzidine (TMB) for H2O2] and spectrophotometric estimation of apoplastic ROS production (O·2– and H2O2). In addition, higher activity of membrane bound NOX (producing O·2– ) and apoplastic class III peroxidase (Prx, producing ·OH) in light grown roots further corroborates the view that apoplastic ROS (initiated with NOX-generated O·2– , which is converted, either spontaneously or by the activity of SOD, to H2O2 and further metabolized by Prx to ·OH that participates in cell wall relaxation required for growth) is instrumental in light-escape growth of roots.N,2-Dihydroxybenzamide (cas: 89-73-6) were involved in the experimental procedure.
N,2-Dihydroxybenzamide(cas: 89-73-6) is a hydroxamic acid that inhibits the activity of p-hydroxybenzoic acid (PHBA) reductase, an enzyme involved in the conversion of PHBA to benzoic acid. Name: N,2-Dihydroxybenzamide The compound has been shown to inhibit mitochondrial membrane potential and mitochondrial functions, leading to cell death.
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