Yang, Qingqing;Zhu, Jianguo;Ma, Fei;Li, Peiwu;Zhang, Liangxiao;Zhang, Wen;Ding, Xiaoxia;Zhang, Qi published 《Quantitative determination of major capsaicinoids in serum by ELISA and time-resolved fluorescent immunoassay based on monoclonal antibodies》. The research results were published in《Biosensors & Bioelectronics》 in 2016.Application In Synthesis of N-Vanillylnonanamide The article conveys some information:
To monitor capsaicinoids in serum on-site, three new monoclonal antibodies (mAbs) were firstly proposed using a conjugate of 4-[(4-hydroxy-3-methoxybenzyl) amino]-4-oxobutanoic acid as the immunogen. Among them, the YQQD8 mAb showed the highest sensitivity and cross-reactivity to major capsaicinoids, such as capsaicin, dihydrocapsaicin and N-vanillylnonanamide. A competitive indirect ELISA (icELISA) and a time-resolved fluorescent immunochromatog. assay (TRFICA) were established based on this mAb. The linear range was 1.1-27.0 ng mL-1 for icELISA and 1.9-62.5 ng mL-1 for TRFICA and the limit of detection (LOD) of TRFICA was 1.5 ng mL-1. To decrease the interference of sample components and increase accuracy, serum samples were diluted four times before assays. As a result, the linear range of serum samples was 4.6-107.9 ng mL-1 for icELISA and 7.6-250.0 ng mL-1 for TRFICA. Both icELISA and TRFICA showed good recoveries (91.0-112.8% for icELISA and 87.6-111.5% for TRFICA) and concordant results in spiked experiments Overall, this is the first report of immunoassay based on the mAbs for quant. determination of major capsaicinoids, and the results demonstrate that both methods can meet the demands of rapid on-site assay for capsaicinoids in serum samples.N-Vanillylnonanamide (cas: 2444-46-4) were involved in the experimental procedure.
N-Vanillylnonanamide(cas:2444-46-4) is also called pelargonic acid vanillylamide or PAVA.Application In Synthesis of N-Vanillylnonanamide Similar to capsaicin, nonivamide can activate the TRPV1 receptor, thus, stimulate the firing rate of dopaminergic neurons in the ventral tegmental area of the brain and to increase the expression of the serotonin receptor gene HTR2A.
Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics