COA of Formula: C22H43NO《UHPLC/GC-TOF-MS metabolomics, MTT assay, and molecular docking studies reveal physostigmine as a new anticancer agent from the ethyl acetate and butanol fractions of Kigelia africana (Lam.) Benth. fruit extracts》 was published in 2021. The authors were Fagbohun, Oladapo F.;Olawoye, Babatunde;Ademakinwa, Adedeji N.;Oriyomi, Olumayowa V.;Fagbohun, Oladoyin S.;Fadare, Olatomide A.;Msagati, Titus A. M., and the article was included in《Biomedical Chromatography》. The author mentioned the following in the article:
Kigelia africana plant is widely used as a herbal remedy in preventing the onset and the treatment of cancer-related infections. With the increase in the research interest of the plant, the specific chem. compound or metabolite that confers its anticancer properties has not been adequately investigated. The Et acetate and butanol fractions of the fruit extracts were evaluated by 2-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazolium bromide assay against four different cell lines, with the Et acetate fraction having inhibition concentration values of 0.53 and 0.42 μM against Hep G2 and HeLa cells, resp. More than 235 phytoconstituents were profiled using UHPLC-TOF-MS, while more than 15 chem. compounds were identified using GC-MS from the fractions. Mol. docking studies revealed that physostigmine, fluazifop, dexamethasone, sulfisomidine, and desmethylmirtazapine could favorably bind at higher binding energies of -8.3, -8.6, -8.2, and -8.1 kcal/mol, resp., better than camptothecin with a binding energy of -7.9 kcal/mol. The results of this study showed that physostigmine interacted well with topoisomerase IIα and had a high score of pharmacokinetic prediction using absorption, distribution, metabolism, excretion, and toxicity profiles, thereby suggesting that drug design using physostigmine as a base structure could serve as an alternative against the toxic side effects of doxorubicin and camptothecin. To complete the study, the researchers used cis-13-Docosenamide (cas: 112-84-5) .
cis-13-Docosenoamide(cas: 112-84-5) was employed as: standard in determination of fatty acid amides in polyethylene packaging film by GC/MS;slip agent for polymers to reduce their friction coefficient and to make films easier to handle.COA of Formula: C22H43NO
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