Month: September 2022

Wen, Jing;Wang, Guo-Liang;Yuan, Hong-Jie;Zhang, Jie;Xie, Hong-Li;Gong, Shuai;Han, Xiao;Tan, Jing-He published 《Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes》 in 2020. The article was appeared in 《Scientific Reports》. They have made some progress in their research.Application of 329-89-5 The article mentions the following:

The developmental competence of IVM porcine oocytes is still low compared with that in their in vivo counterparts. Although many studies reported effects of glucose metabolism (GM) on oocyte nuclear maturation, few reported on cytoplasmic maturation. Previous studies could not differentiate whether GM of cumulus cells (CCs) or that of cumulus-denuded oocytes (DOs) supported oocyte maturation. Furthermore, species differences in oocyte GM are largely unknown. Our aim was to address these issues by using enzyme activity inhibitors, RNAi gene silencing and special media that could support nuclear but not cytoplasmic maturation when GM was inhibited. The results showed that GM in CCs promoted pig oocyte maturation by releasing metabolites from both pentose phosphate pathway and glycolysis. Both pyruvate and lactate were transferred into pig DOs by monocarboxylate transporter and pyruvate was further delivered into mitochondria by mitochondrial pyruvate carrier in both pig DOs and CCs. In both pig DOs and CCs, pyruvate and lactate were utilized through mitochondrial electron transport and LDH-catalyzed oxidation to pyruvate, resp. Pig and mouse DOs differed in their CC dependency for glucose, pyruvate and lactate utilization. While mouse DOs could not, pig DOs could use the lactate-derived pyruvate. The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Application of 329-89-5 is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

De Abrew, K. Nadira;Shan, Yuqing K.;Wang, Xiaohong;Krailler, Jesse M.;Kainkaryam, Raghunandan M.;Lester, Cathy C.;Settivari, Raja S.;LeBaron, Matthew J.;Naciff, Jorge M.;Daston, George P. published 《Use of connectivity mapping to support read across: A deeper dive using data from 186 chemicals, 19 cell lines and 2 case studies》 in 2019. The article was appeared in 《Toxicology》. They have made some progress in their research.Product Details of 329-89-5 The article mentions the following:

The authors previously demonstrated that the Connectivity Map (CMap) (Lamb et al., 2006) concept can be successfully applied to a predictive toxicol. paradigm to generate meaningful MoA-based connections between chems. (De Abrew et al., 2016). Here the authors expand both the chem. and biol. (cell lines) domain for the method and demonstrate two applications, both in the area of read across. In the first application the authors demonstrate CMap’s utility as a tool for testing biol. relevance of source chems. (analogs) during a chem. led read across exercise. In the second application CMap can be used to identify functionally relevant source chems. (analogs) for a structure of interest (SOI)/target chem. with minimal knowledge of chem. structure. Finally, the authors highlight four factors: promiscuity of chem., dose, cell line and timepoint as having significant impact on the output. The authors discuss the biol. relevance of these four factors and incorporate them into a work flow.6-Aminonicotinamide (cas: 329-89-5) were involved in the experimental procedure.

6-Aminonicotinamide (cas:329-89-5)Product Details of 329-89-5 induces apoptosis in tumor cells. It is clinically used in disseminated neoplastic disease. It also acts as 6-phosphogluconate dehydrogenase inhibitor. It aids in the treatment of psoriasis. It is used as cancer chemotherapeutic drug in animals.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Oburoglu, Leal;Tardito, Saverio;Fritz, Vanessa;de Barros, Stephanie C.;Merida, Peggy;Craveiro, Marco;Mamede, Joao;Cretenet, Gaspard;Mongellaz, Cedric;An, Xiuli;Klysz, Dorota;Touhami, Jawida;Boyer-Clavel, Myriam;Battini, Jean-Luc;Dardalhon, Valerie;Zimmermann, Valerie S.;Mohandas, Narla;Gottlieb, Eyal;Sitbon, Marc;Kinet, Sandrina;Taylor, Naomi published 《Glucose and Glutamine Metabolism Regulate Human Hematopoietic Stem Cell Lineage Specification》. The research results were published in《Cell Stem Cell》 in 2014.Related Products of 329-89-5 The article conveys some information:

The metabolic state of quiescent hematopoietic stem cells (HSCs) is an important regulator of self-renewal, but it is unclear whether or how metabolic parameters contribute to HSC lineage specification and commitment. Here, we show that the commitment of human and murine HSCs to the erythroid lineage is dependent upon glutamine metabolism HSCs require the ASCT2 glutamine transporter and active glutamine metabolism for erythroid specification. Blocking this pathway diverts EPO-stimulated HSCs to differentiate into myelomonocytic fates, altering in vivo HSC responses and erythroid commitment under stress conditions such as hemolytic anemia. Mechanistically, erythroid specification of HSCs requires glutamine-dependent de novo nucleotide biosynthesis. Exogenous nucleosides rescue erythroid commitment of human HSCs under conditions of limited glutamine catabolism, and glucose-stimulated nucleotide biosynthesis further enhances erythroid specification. Thus, the availability of glutamine and glucose to provide fuel for nucleotide biosynthesis regulates HSC lineage commitment under conditions of metabolic stress. To complete the study, the researchers used 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Related Products of 329-89-5 is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Ma, Siguang;Zhou, Xinpeng;Jahan, Mohammad Shah;Guo, Shirong;Tian, Mimi;Zhou, Ranran;Liu, Hongyun;Feng, Bingjie;Shu, Sheng published 《Putrescine regulates stomatal opening of cucumber leaves under salt stress via the H₂O₂-mediated signaling pathway》 in 2022. The article was appeared in 《Plant Physiology and Biochemistry (Issy-les-Moulineaux, France)》. They have made some progress in their research.SDS of cas: 89-73-6 The article mentions the following:

The stomatal aperture is imperative for photosynthesis in higher plants. The function of polyamines (PAs) in stomatal regulation under a stressful environment has not been fully determined In this study, we demonstrated the mechanism by which putrescine (Put) regulates stomatal changes in cucumber leaves under salt stress. The results showed that foliar application of Put alleviated the decrease of stomatal aperture and photosynthesis caused by salt stress and promoted plant growth. Exogenous Put caused a significant increase in endogenous PAs and hydrogen peroxide (H₂O₂) levels by 105.43% and 27.97%, resp., while decreased abscisic acid (ABA) content by 67.68% under salt stress. However, application of inhibitors of aminoguanidine hydrochloride (AG), 1, 8-diaminooctane (1, 8-DO), diphenyleneiodonium chloride (DPI) and salicylhydroxamic acid (SHAM) upregulated the 9-cis-cyclocarotenoid dioxygenase (NCED) gene and downregulated the reduced glutathione synthetase (GSHS) gene. These inhibitors also decreased the stomatal aperture, levels of H₂O₂ and reduced glutathione (GSH), but increased the ABA content under salt stress and Put treatment conditions. The order of influence is AG > 1, 8-DO > DPI > SHAM. However, Put-induced downregulation of ABA content and upregulation of GSH content under salt stress were effectively blocked by N, N’-dimethylthiourea (DMTU, H₂O₂ scavenger) and 1-chloro-2,4-dinitrobenzene (CDNB, GSH scavenger) treatments. Taken together, these results suggest that Put induced the formation of H₂O₂ signaling mediates the degradation of PAs by diamine oxidase (DAO), increasing GSH content and inhibiting the accumulation of ABA in leaves, thereby promoting stomatal opening in salt-stressed cucumber leaves.N,2-Dihydroxybenzamide (cas: 89-73-6) were involved in the experimental procedure.

N,2-Dihydroxybenzamide(cas: 89-73-6) can be used:To prepare phenylboronic acid-based bioconjugates for chromatographic applications;As a ligand to synthesize Fe(III), Cu(II), Ni(II) and Zn(II) complexes.SDS of cas: 89-73-6

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Formula: C22H43NOIn 2021, Cui, Huan;Gao, Weichang;Lin, Yechun;Zhang, Jie;Yin, Runsheng;Xiang, Zhangmin;Zhang, Song;Zhou, Shuping;Chen, Wensheng;Cai, Kai published 《Development of microwave-assisted extraction and dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry for determination of organic additives in biodegradable mulch films》. 《Microchemical Journal》published the findings. The article contains the following contents:

Organic additives are extensively used as ingredients in biodegradable mulch films. They are swiftly released into the environment, which may have an ecotoxicol. impact on plant growth and development, as well as on soil microbial community abundance and function. Herein, a method based on the application of microwave-assisted extraction (MAE) and dispersive liquid-liquid microextraction (DLLME) was developed to analyze eighty organic additives by gas chromatog.-mass spectrometry in poly(butylene adipate terephthalate) (PBAT) biodegradable mulch films. This was a comprehensive study, including the identification of organic additives, optimization of MAE and DLLME methods, anal. of isocyanate conversion, and evaluation of the matrix effect (ME). Under the optimized exptl. conditions, this method exhibited excellent detection capabilities for organic additives, except for 5 kinds of isocyanates and their reaction products, with coefficients of determination R² > 0.999 and lack of fit P > 0.05 in linear regression parameters. A negligible ME was observed The relative recoveries were 93.0-109.8%, and the repeatability and reproducibility varied within the ranges of 2.06-8.76% and 2.38-10.23%, resp. The limits of detection and limits of quantitation were 0.0008-0.0586μg g^-1 and 0.003-0.195μg g^-1, resp. The developed method was further successfully applied to the anal. of organic additives in PBAT biodegradable mulch films from four different manufacturers. Interestingly, the Venn diagram and principal component anal. showed that different manufacturing origins display obvious characteristic differences in the organic additive types and concentrationscis-13-Docosenamide (cas: 112-84-5) were involved in the experimental procedure.

cis-13-Docosenoamide(cas: 112-84-5) is a primary fatty amide resulting from the formal condensation of the carboxy group of erucic acid with ammonia.Formula: C22H43NO It is commonly used as a slip additive in the plastic manufacturing industry.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Le Coz, Florian;Suzuki, Noriyuki;Nagahori, Hirohisa;Omori, Takashi;Saito, Koichi published 《Hand1-Luc embryonic stem cell test (Hand1-Luc EST): a novel rapid and highly reproduciblein vitro test for embryotoxicity by measuring cytotoxicity and differentiation toxicity using engineered mouse ES cells》 in 2015. The article was appeared in 《Journal of Toxicological Sciences》. They have made some progress in their research.Application In Synthesis of 6-Aminonicotinamide The article mentions the following:

The embryonic stem cell test (EST) is a promising alternative method for evaluating embryotoxicity of test chems. by measuring cytotoxicity and differentiation toxicity using mouse ES cells. Differentiation toxicity is analyzed by microscopically counting the beating of embryonic bodies after 10 days of culture. However, improvements are necessary to reduce the laborious manipulations involved and the time required to obtain results. We have previously reported the successful stable transfection of ES cells (ES-D3) with the heart and neural crest derivatives expressed transcript 1 (Hand1) gene and the establishment of a 96-well multi-plate-based new EST with luciferase reporter assay 6 days after treatment with test chems. Now, we propose an even more rapid and easier EST, named Hand1-Luc EST. We established another cell line to monitor the Hand1 gene expression via a luciferase reporter gene. By mRNA anal. and luciferase assay, we examined in detail the luciferase activity during cell differentiation, which allowed us to reduce the time of measurement from day 6 to day 5 (120 h). Furthermore, the protocol was improved, with, among others, the measurement of cytotoxicity and differentiation toxicity taking place in the same 96-well round bottom plate instead of two different plates. With the pos. control, 5-fluorouracil (5-FU), and 9 test chems., data with high reproducibility and very low variation (CV < 50%) in the relevant endpoints were obtained. This study shows that the Hand1-Luc EST could provide an accurate and sensitive short-term test for prediction of embryotoxicants by measuring cytotoxicity and differentiation toxicity from the same sample. The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Application In Synthesis of 6-Aminonicotinamide is an inhibitor of the NADP+-dependent enzyme, PGD (6-phosphogluconate dehydrogenase). Studies have also shown that 6-aminonicotinamide induces apoptosis in tumor cells and causes glial cell degeneration.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Guo, Yu-Hua;Yu, You-Zhu;Shen, Yan-Hong;Yang, Li-Guo;Liu, Na-Na;Zhou, Zhong-Yuan;Niu, Yong-Sheng published 《”Three-in-One” Structural-Building-Mode-Based Ti₁₆-Type Titanium Oxo Cluster Entirely Protected by the Ligands Benzoate and Salicylhydroxamate》 in 2022. The article was appeared in 《Inorganic Chemistry》. They have made some progress in their research.Product Details of 89-73-6 The article mentions the following:

Titanium oxo clusters (TOCs) with accurate mol. structures have potential applications in photocatalysis, such as photocatalytic degradation, hydrogen production, and water oxidation The hydrolytic stability and light absorption ability of TOCs have important impacts on photocatalysis, where the selection of peripheral organic ligands plays a significant role. In this regard, salicylhydroxamic acid (abbreviated as H₃L) attracts our attention, acting as a ligand for its multidentate and dye-functional features, which can increase the hydrolytic stability and broaden light absorption for TOCs. Herein, two TOCs were solvothermally synthesized and structurally characterized using H₃L, formulated as [Ti₈(μ₂-O)₂(μ₃-O)₂(OⁱPr)₁₂(L)₄]·2CH₃CN (1) and [Ti₁₆(μ₂-O)₁₀(μ₃-O)₄(PhCOO)₁₄(L)₆(HL)₂]·4CH₃CN·2ⁱPrOH (2). Complex 2 was obtained by adding excessive benzoic acid over the reaction system of 1, resulting in enhanced hydrolytic stability via the replacement of all alkoxy ligands by multidentate ligands for protection. Interestingly, for the first time, the “three-in-one” structural building mode with {Ti₆} + {Ti₄} + {Ti₆} by the common subunits in 2 was observed among all reported TOCs. Moreover, complex 2 can strongly absorb visible light reaching up to 700 nm and exhibit obvious activity for the photodegradation of methyl orange. The experimental procedure involved many compounds, such as N,2-Dihydroxybenzamide (cas: 89-73-6) .

N,2-Dihydroxybenzamide(cas: 89-73-6) has also been shown to be active against wild-type strains of Candida glabrata, but not against resistant mutants. Product Details of 89-73-6 This drug may have therapeutic potential for bone cancer and metabolic disorders such as obesity.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

COA of Formula: C22H43NOIn 2022, Chen, Zhi-Feng;Lin, Qin-Bao;Dong, Ben;Zhong, Huai-Ning;Wang, Zhi-Wei published 《Comparison of the ability of UV-Vis and UPLC-Q-TOF-MS combined with chemometrics to discriminate recycled and virgin polyethylene》. 《Journal of Hazardous Materials》published the findings. The article contains the following contents:

A growing attention is attracted to the use of recycled plastics as food contact materials, and its chem. safety research and discrimination approach are indispensable. In current study, UV-visible spectrometry (UV-Vis) and ultra-performance liquid chromatog. quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) were used to provide spectral and mass fingerprinting for polyethylene (PE). Coupling with chemometrics, two methods were developed to discriminate recycled and virgin PE. UV-Vis combined with chemometrics could be a more accessible, simpler and faster approach. 237-331 nm in UV spectrum was regarded as marker region selected by orthogonal partial least-squares discrimination anal. (OPLS-DA) and the accuracy of both calibration and validation set could reach 100% in linear discrimination anal. (LDA) based on this region. Besides, 2314 ions were detected by UPLC-Q-TOF-MS and processed by MS-DIAL. 48 candidate chems. were identified, including ketone, esters, carboxylic acid, alcs. and phenols, amine, nitriles, aldehydes and others. Possible origins of these compounds could be classified as plastic, food, drug, cosmetics and pesticide related. Many of these compounds are highly toxic, especially pesticide related, indicating that recycling in closed loop or sorting by the recycled plastic articles is very necessary if the recycled PE is going to be used as food contact material. To complete the study, the researchers used cis-13-Docosenamide (cas: 112-84-5) .

cis-13-Docosenoamide(cas: 112-84-5) is a primary fatty amide resulting from the formal condensation of the carboxy group of erucic acid with ammonia.COA of Formula: C22H43NO It has a role as a human metabolite, a rat metabolite, a mammalian metabolite, a plant metabolite and an EC 3.1.1.7 (acetylcholinesterase) inhibitor.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Zhang, Xin;Zheng, Yuanrong;Zhou, Ran;Ma, Ming published 《Comprehensive identification of molecular profiles related to sensory and nutritional changes in Mongolian cheese during storage by untargeted metabolomics coupled with quantification of free amino acids》 in 2022. The article was appeared in 《Food Chemistry》. They have made some progress in their research.Application of 112-84-5 The article mentions the following:

Non-targeted metabolomics was used to study metabolites with low mol. weight which may contribute to quality deterioration of Mongolian cheese during storage. Microbiol. anal., pH, FAAs (free amino acids), volatile compounds, and sensory evaluation of the cheese during storage were also studied. A total of 278 metabolites were identified in Mongolian cheese, of which 51 metabolites were used as differential metabolites, including amino acids, peptides, organic acids, lipids, and carbohydrates. Bitter amino acids, bitter peptide (Phe-Ile), and organic acids (sinapic acid, butyric acid) increased during storage. Metabolic pathway anal. showed that differential metabolites were mainly related to amino acid metabolism, such as β-alanine metabolism and glycine, serine, and threonine metabolism Moreover, accompanied with the increased contents of short-chain fatty acids, 2-undecanone and Et esters, strength of odor and unpleasant smell increased but overall acceptability decreased during Mongolian cheese storage. This research provides suitable strategies for quality control of Mongolian cheese during shelf life.cis-13-Docosenamide (cas: 112-84-5) were involved in the experimental procedure.

cis-13-Docosenoamide(cas: 112-84-5) was employed as: standard in determination of fatty acid amides in polyethylene packaging film by GC/MS;slip agent for polymers to reduce their friction coefficient and to make films easier to handle.Application of 112-84-5

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Electric Literature of C6H7N3O《Metabolic interaction of hydrogen peroxide and hypoxia in zebrafish fibroblasts》 was published in 2020. The authors were Dikova, Valentina;Vorhauser, Julia;Geng, Anne;Pelster, Bernd;Sandbichler, Adolf Michael, and the article was included in《Free Radical Biology & Medicine》. The author mentioned the following in the article:

Cells require oxygen for aerobic metabolism, which may also result in the production of reactive oxygen species (ROS) as a byproduct. Under low oxygen conditions, ROS formation has been reported to either increase or decrease. We addressed this physiol. response for the first time in zebrafish embryonic fibroblasts (Z3) and used a hydrogen peroxide (H₂O₂)-specific fluorescent protein (roGFP2-Orp1) either targeted to the mitochondria or expressed in the cytosol. Microfluidic live-cell imaging measurements showed that oxygen deprivation in Z3 cells results in decreased or stable H₂O₂ levels within the mitochondria or the cytosol, resp., and that the reductive shift recorded in the mitochondrial matrix is directly dependent on oxygen concentration The response was accompanied by a transient increase in extracellular acidification rate (ECAR) and a lower cellular reducing potential as assessed by the viability stain alamarBlue. Complex I and III inhibition with Rotenone and Antimycin A led to H₂O₂ production under normoxia but these inhibitors were not able to avert the reductive shift under hypoxia. Only by system-wide inhibition of flavin-containing oxidases with Diphenyleneiodonium (DPI) were we able to decrease the reductive shift, while selective inhibition of NADPH oxidases with the inhibitor Apocynin had no effect on the hypoxia response. Since DPI also led to a strong increase in ECAR we found that, in order to keep the cytosolic H₂O₂ levels stable, glycolytic metabolism was of fundamental importance. According to our experiments with the glucose-6-phosphate dehydrogenase inhibitor 6-Aminonicotinamide, this was attributable to the pentose phosphate pathway producing reducing equivalent required for ROS degradation The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Electric Literature of C6H7N3O is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics