Application of 135-57-9, Catalysts allow a reaction to proceed via a pathway that has a lower activation energy than the uncatalyzed reaction. 135-57-9, Name is Bis(2-benzamidophenyl) Disulfide, SMILES is O=C(NC1=CC=CC=C1SSC2=CC=CC=C2NC(C3=CC=CC=C3)=O)C4=CC=CC=C4, belongs to amides-buliding-blocks compound. In a article, author is Qin, Y., introduce new discover of the category.
Functionalized antibodies are an indispensable resource for diagnosis, therapy and as a research tool for chemical biology. However, simpler and better methodologies are often required to improve the labeling of antibodies in terms of selectivity and scalability. Herein, we report the development of an easily available chemical reagent that allows site-directed labeling of native human IgG1 antibodies in good yield and mono-labeling selectivity. The salicylaldehyde moiety of the reagent reacts with surface exposed lysine residues to transiently form an iminium ion, and this positions a semi-reactive ester in proximity of a second lysine residue that reacts with the ester to form an amide. Interestingly, it appears that the formation of the iminium ion also has a significant activating effect of the ester. We use flow cytometry and bio-layer interferometry to confirm that the labeled antibodies retain antigen binding.
Application of 135-57-9, Because enzymes can increase reaction rates by enormous factors and tend to be very specific, typically producing only a single product in quantitative yield, they are the focus of active research.you can also check out more blogs about 135-57-9.
Reference:
Amide – Wikipedia,
,Amide – an overview | ScienceDirect Topics