87-32-1, Name is N-Acetyl-DL-tryptophan, molecular formula is C13H14N2O3, COA of Formula: C13H14N2O3, belongs to amides-buliding-blocks compound, is a common compound. In a patnet, author is Trainor, Kyle, once mentioned the new application about 87-32-1.
Protein crosslinking improves the thermal resistance of plastocyanin immobilized on a modified gold electrode
Increasing the thermal stability of immobilized proteins is a motivating goal for improving the performance of electrochemical biodevices. In this work, we propose the immobilization of crosslinked plastocyanin from the thermophilic cyanobacterium Phormidium laminosum by simultaneous incubation of a mixture of plastocyanin and the coupling reagents. The thermal stability of the so built covalently immobilized protein films has been assessed by cyclic voltammetry in the 0-90 degrees C temperature range and has been compared to that of physisorbed films. It is shown that the protein loss along a thermal cycle is significantly reduced in the case of the crosslinked films, whose redox properties remain unaltered along a cyclic heating-cooling thermal scan, and can withstand the contact with 70 degrees C solutions for four hours. Comparison of thermal unfolding curves obtained by circular dichroism spectroscopy of both free and crosslinked protein confirms the improved thermic resistance of the crosslinked plastocyanin. Notably, the electron transfer thermodynamics of physisorbed and crosslinked plastocyanin films are quite similar, suggesting that the formation of intra- and inter-protein amide bonds do not affect the integrity and functionality of the copper redox centers. UV-Vis absorption and circular dichroism measurements corroborate that protein crosslinking does not alter the coordination geometry of the metal center. (C) 2018 Elsevier B.V. All rights reserved.
I hope this article can help some friends in scientific research. I am very proud of our efforts over the past few months and hope to 87-32-1 help many people in the next few years. COA of Formula: C13H14N2O3.