Chemistry can be defined as the study of matter and the changes it undergoes. You’ll sometimes hear it called the central science because it is the connection between physics and all the other sciences, starting with biology. 1185-53-1, Name is Tris hydrochloride, molecular formula is , belongs to amides-buliding-blocks compound. In a document, author is Cobos, Ana, Recommanded Product: 1185-53-1.
A Computationally Designed Peptide Derived from Escherichia coli as a Potential Drug Template for Antibacterial and Antibiofilm Therapies
Computer-aided screening of antimicrobial peptides (AMPs) is a promising approach for discovering novel therapies against multidrug-resistant bacterial infections. Here, we functionally and structurally characterized an Escherichia coli-derived AMP (EcDBS1R5) previously designed through pattern identification [alpha-helical set (KK[ILV]((3))[AILV])], followed by sequence optimization. EcDBS1R5 inhibited the growth of Gram-negative and Gram-positive, susceptible and resistant bacterial strains at low doses (2-32 mu M), with no cytotoxicity observed against non-cancerous and cancerous cell lines in the concentration range analyzed (<100 mu M). Furthermore, EcDBS1R5 (16 mu M) acted on Pseudomonas aeruginosa pre-formed biofilms by compromising the viability of biofilm-constituting cells. The in vivo antibacterial potential of EcDBS1R5 was confirmed as the peptide reduced bacterial counts by two-logs 2 days post-infection using a skin scarification mouse model. Structurally, circular dichroism analysis revealed that EcDBS1R5 is unstructured in hydrophilic environments, but has strong helicity in 2,2,2-trifluoroethanol (TFE)/water mixtures (v/v) and sodium dodecyl sulfate (SDS) micelles. The TFE-induced nuclear magnetic resonance structure of EcDBS1R5 was determined and showed an amphipathic helical segment with flexible termini. Moreover, we observed that the amide protons for residues Met2-Ala8, Arg10, Ala13-A1a16, and Trp19 in EcDBS IRS are protected from the solvent, as their temperature coefficients values are more positive than -4.6 ppb center dot K-1. In summary, this study reports a novel dual-antibacterial/antibiofilm alpha-helical peptide with therapeutic potential in vitro and in vivo against clinically relevant bacterial strains. If you are hungry for even more, make sure to check my other article about 1185-53-1, Recommanded Product: 1185-53-1.