Chemistry is the experimental and theoretical study of materials on their properties at both the macroscopic and microscopic levels. 62009-47-6, Name is 2-Aminomalonamide, molecular formula is C3H7N3O2. In an article, author is Chen, Jin-Ming,once mentioned of 62009-47-6, SDS of cas: 62009-47-6.
Gene ontology analysis of expanded porcine blastocysts from gilts fed organic or inorganic selenium combined with pyridoxine
Background: Gene ontology analysis using the microarray database generated in a previous study by this laboratory was used to further evaluate how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of expanded porcine blastocysts. Data were generated from 18 gilts randomly assigned to one of three experimental diets (n=6 per treatment): i) basal diet without supplemental Se or pyridoxine (CONT); ii) CONT +0.3 mg/kg of Na-selenite and 10mg/kg of HCl-pyridoxine (MSeB(6)10); and iii) CONT +0.3mg/kg of Se-enriched yeast and 10 mg/kg of HCl-pyridoxine (OSeB(6)10). All gilts were inseminated at their fifth post-pubertal estrus and euthanized 5days later for embryo harvesting. Differential gene expression between MSeB(6)10 vs CONT, OSeB(6)10 vs CONT and OSeB(6)10 vs MSeB(6)10 was performed using a porcine embryo-specific microarray. Results: There were 559, 2458, and 1547 differentially expressed genes for MSeB(6)10 vs CONT, OSeB(6)10 vs CONT and OSeB(6)10 vs MSeB(6)10, respectively. MSeB(6)10 vs CONT stimulated 13 biological processes with a strict effect on RNA binding and translation initiation. OSeB(6)10 vs CONT and OSeB(6)10 vs MSeB(6)10 impacted 188 and 66 biological processes, respectively, with very similar effects on genome stability, ceramide biosynthesis, protein trafficking and epigenetic events. The stimulation of genes related with these processes was confirmed by quantitative real-time RT-PCR. Conclusions: Gene expression of embryos from OSeB(6)10 supplemented gilts was more impacted than those from MSeB(6)10 supplemented gilts. Whereas maternal OSeB(6)10 supplementation influenced crucial aspects of embryo development, maternal MSeB(6)10 supplementation was restricted to binding activity.
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